Long-Evans rats are thought to come from crosses between Wistar females and a wild Norway rat (Pass and Freeth 1993). AMI model is usually judged by electrocardiographic characteristics. However, 2 mg/kg/day would be a LOAEL for the hyaline droplet-related nephropathy that was evident in male rats at all TNB dose levels. Gestation for Sprague Dawley on an average is a cycle of 22 days (Sprague Dawley, 2006). Sprague-Dawley rats maintained on diet containing 265 ppm cobalt for 98 days showed degenerative changes in the testes considered to be secondary to hypoxia (Mollenhauer et al., 1985). Testes and epididymides were weighed and examined histopathologically. In order to examine the degree of variability of an outbred stock obtained from various sources, Sprague Dawley (SD) rat lines obtained from two sources were evaluated for their growth characteristics. Pedigo and Vernon (1993) also reported that treatment of male mice with cobalt chloride (400 ppm in the diet during 10 weeks) increased preimplantation losses in the dominant lethal assay. The last injection was given 24 h prior to sacrifice. These rats typically have increased tail to body length ratio compared with Wistar rats. Sprague–Dawley rats, born in the laboratory.were maintained under conditions of controlled temperature and lighting (12 h-12 h) with free access to food and water. Δ4 was reduced to T [7], repurified on a second celite column chromatography and measured as T. Specific radioimmunoassays, described in detail elsewhere [7, 24, 25], were used to determine the plasma concentrations of T, Δ4 and OHP in both species. The current study aims to investigate the influence of five rare earth elements (REEs) (i.e., lanthanum (La), cerium (Ce), praseodymium (Pr), neodymium (Nd), and gadolinium (Gd)) on the growth of Sprague–Dawley (SD) rats, and to explore the accumulation characteristics of REEs in tissues and organs with different doses as well as the detoxification and elimination of high-dose REEs. injections of 20 mg/kg dissolved in peanut oil in 12 h intervals. including alloxan- induced diabetic Sprague- Dawley rats. The tissues presented in this Atlas are based on a limited number of animals examined. Characteristics of Open Field Behavior of Wistar and Sprague-Dawley Rats Yuzo ASANO Safety Research Laboratory, Tanabe Seiyaku Co., Ltd., Kashima 3-16-89, Yodogawa-ku, Osaka 532, Japan (Received 31 January 1986/Accepted 2 May 1986) The open field test (OFT) was carried out on Wistar and Sprague-Dawley rats Blood was collected by decapitation from 3000 rats between 12.00 and 13.00. Treatment with 400 ppm cobalt chloride in the diet for 10 weeks induced seminiferous tubule degeneration in mice (Anderson et al., 1992). Sprague–Dawley rat pups of postnatal day (P) 10 or 20 were subjected to generalized and sustained convulsions (>20 min duration, a model for status epilepticus, SE), provoked by i.p. However, moderate DR did improve survival by delaying the onset and progression of spontaneous degenerative disease and tumors in Sprague-Dawley rats ( Keenan et al. The magnification listed in each figure legend represents the objective magnification used when capturing the digital image, as indicated by the NDP.View II software. MATERIALS AND METHODS Four hundred eighty 5-week old Sprague- Dawley, CD, outbred albino rats, 240 males Almost all laboratory and domestic pet rats belong to a single species, the Norway rat (Rattus norvegicus). Xiaokun Li, ... Xiaokun Li, in Fibroblast Growth Factors, 2018. Bilateral adrenalectomy, ovariectomy and orchidectomy were carried out aseptically under anesthesia of Nembutol. Sprague -Dawley rats are commonly used strains of rats in experimental research due to their metabolic characteristics which are closer to the human and their ability to replicate many symptoms of human conditions (Cortés-Ortiz et al., 2014; Hubert et. In Atlas of Histology of the Juvenile Rat, 2016. Sprague Dawleys are unable to vomit due to the esophagus entering the stomach. Dogs exposed at the high dose showed neurotoxic symptoms such as salivation, hypoactivity, trembling, hyperesthesia, myoclonia, groaning, and prostration before death. In a second study, male Sprague Dawley rats [Crl:CD(SD)], weighing 150–200 g, were obtained from Charles River Laboratories (Raleigh, NC), and the banding surgery was performed at GSK according to the methods stated above with the exception of the use … Rats that underwent adrenalectomy or adrenalectomy and castration were given 0.9% NaCl solution in place of water. Hence, this study aimed to investigate the anti-obesity activity of Elateriospermum tapos (E. tapos) shell extract in obesity induced Sprague Dawley rats. Sprague Dawley rats were used to examine the reproductive impacts of dietary exposure to TNB [34]. Sprague–Dawley rats, born in the laboratory.were maintained under conditions of controlled temperature and lighting (12 h-12 h) with free access to food and water. The rat is the most commonly used rodent species for toxicology studies. For each day of life 6–8 pools were made. Decreases in sperm motility and/or increased abnormal sperm were noted in mice, but not in rats, exposed to 3 mg/m3 or more in 13-week inhalation studies with cobalt sulfate (National Toxicology Program, 1991). A left thoracotomy was performed through the fourth intercostal space using a sterile technique. Sprague–Dawley rats of both sexes, 10–12 weeks old weighing 125–300 g, were allowed access to commercial rat chow pellets and drinking water ad libitum. The Hsd:Sprague Dawley ® SD rat has a wide array of historical research use including in the field of cardiology. The relationship of this change with other causes of endocardial thickening is discussed, and the significance of these alterations is assessed. In this study inter assay variations were 6.5%, for T, 6.9%, for Δ4 and 8.4% for OHP. Ten animals/sex/group received 0, 70, 400, or 800 mg TNB/kg diet, levels calculated by the authors to result in average doses of 0, 3, 23, and 51 mg/kg/day in males and 0, 4, 30, and 60 mg/kg/day in females (0, 8, 55, and 110 mg/kg/day during lactation). For collection of a few selected tissues, young pups at PND 1, 7, and 14 were perfused with NBF via intracardiac instillation of fixative. F0 female rats were necropsied at termination with pieces of spleen, kidney, and liver processed for histopathological evaluation. Navas, in Encyclopedia of Toxicology (Third Edition), 2014. Pathological studies were undertaken on 106 mammary tumours (89 benign, 17 malignant) appearing spontaneously in 95 normal female Sprague-Dawley rats which were killed at … HSIA, ... W. VOIGT, in Hormonal Steroids: Proceedings of the Sixth International Congress on Hormonal Steroids, 1983. The objective of this study was to evaluate the toxic characteristics of Rauvolfia tetraphylla leaf extract in Sprague dawley rats after treatment for minimum 7 days by oral route. Striatal RTI-55 radioactivities in the caudate nucleus are estimated using Amersham autoradiographic 125I-labeled microscales as the exposure standards. Background and aims: Daily nutrient intake and growth of male Sprague-Dawley (SD) rats fed compositionally different diets were monitored over 114 weeks to determine whether rats fed ad libitum (AL) or diet restricted (DR) followed normal growth parameters. Sexual dimorphism in various tissues is presented in the text, and illustrated where possible. Sprague Dawleys are unable to vomit due to the esophagus entering the stomach. The average litter size of the Sprague Dawley rat is 10.5. We use cookies to help provide and enhance our service and tailor content and ads. The aim of this study was to ascertain experimentally, through the model of altering the early mother-infant bond and the late social bond through isolation, the effect on the histologic characteristics of the liver in adult Sprague-Dawley rats subjected to intermittent chronic stress. The Sprague-Dawley strain came from crosses between Wistar females and a "hybrid" male whose origins are unknown. Whole brain sections were used for (i) quantitative radioligand binding autoradiography, (ii) in situ hybridization, and (iii) [35S]GTPγS autoradiography, to assess the properties of hippocampal and neocortical muscarinic receptors. Hence, this study aimed to investigate the anti-obesity activity of Elateriospermum tapos (E. tapos) shell extract in obesity induced Sprague Dawley rats. Gunda Reddy, Marc A. Williams, in Wildlife Toxicity Assessments for Chemicals of Military Concern, 2015. Neurological signs, for example, head tilt and loss of equilibrium, were observed in lactating dams. The strain was created by R. W. Dawley in 1925, from a hooded male hybrid of unknown origin and an albino female (probably Wistar), and was crossed with the female’s progeny for 7 generations.The SPRAGUE DAWLEY® rat is an albino outbred rat with an elongated head and a … outbred stock obtained from various sources, Sprague Dawley (SD) rat lines obtained from two sources were evaluated for their growth characteristics. Microsomes were prepared as described previously (8) and used immediately after preparation Spectral measurements were performed in a Perkin Elmer 356 two wavelength double beam spectrophotometer. The rats … 1995a and 1995b). The Sprague-Dawley (SD) is an outbred rat, first generated by Robert S. Dawley during the 1920s by breeding Wistar rats to hybrids of laboratory derived and wild stocks. The SD rats from Charles River laboratories (CRL) and Harlan Laboratories (HAR) were monitored for weight gain from the age of 6 weeks to 24 weeks. These SD rats were fasted but allowed free access to water for 12 h prior to each experiment. The doses, as calculated by the authors, were 0, 2, 9, and 19 mg/kg/day for males and 0, 3, 14, and 29 mg/kg/day for females. This study was conducted to investigate absorption characteristics of zinc glycine chelate (Zn–Gly) by evaluating tissues zinc status and the expression of zinc transporters in rats. Metyrapone binding was studied as decribed previously (9). 2B). These findings suggest that clues from rats of familiar strains influence helping behavior, whereas clues from rats of unfamiliar strains are largely ignored. These findings suggest that clues from rats of familiar strains influence helping behavior, whereas clues from rats of unfamiliar strains are largely ignored. These rats typically have increased tail to body length ratio compared with Wistar rats. However, moderate DR did improve survival by delaying the onset and progression of spontaneous degenerative disease and tumors in Sprague-Dawley rats ( Keenan et al. The SD rats from Charles River laboratories (CRL) and Harlan Laboratories (HAR) were monitored for weight gain from the age of 6 weeks to 24 weeks. Background and aims: Daily nutrient intake and growth of male Sprague-Dawley (SD) rats fed compositionally different diets were monitored over 114 weeks to determine whether rats fed ad libitum (AL) or diet restricted (DR) followed normal growth parameters. Animals were divided into subgroups to accommodate a number of premating, postmating, and recovery dosing regimens. Reduced sperm motility and/or increased abnormal sperm were noted in mice, but not in rats, exposed to 3 mg/m 3 or higher in 13-week inhalation studies with cobalt sulfate ( NTP, 2011a ). Then the SD rats were anesthetized with an intraperitoneal injection of chloral hydrate (350 mg/kg body weight). Methods: An automated haematology analyzer was used to determine haematological indices of whole blood from individual in 20 each of male and female inbred Sprague Dawley rats. [34], there were no adverse effects of TNB on mating or fertility, gestation length, sex ratio, or the number of offspring/litter. In this single-dose study, the metabonomic profile changes in the 48 h after orally administrated 3-MCPD dipalmitate at 1600 mg/kg BW to Sprague–Dawley (SD) rats were determined with liquid chromatography (LC) coupled with mass spectrometry (MS) system. Sham control groups are treated intranigrally with either 1 μl Ringer's solution or 1.05 μl 0.3 M NaOH. Characteristics of Open Field Behavior of Wistar and Sprague-Dawley Rats Yuzo ASANO Safety Research Laboratory, Tanabe Seiyaku Co., Ltd., Kashima 3-16-89, Yodogawa-ku, Osaka 532, Japan (Received 31 January 1986/Accepted 2 May 1986) The open field test (OFT) was carried out on Wistar and Sprague-Dawley rats between the ages of 3 to 20 weeks. For phenobarbital pretreatment the animals received 3 i.p. By contrast, outbred populations are used when identical genotypesare unnecessary or a population with genetic variation is required, and these rats are usually referred to as stocks rather than … They were housed under standard conditions of controlled temperature, humidity and light and dark cycles. Perhaps the most novel findings of the study related to reproductive performance, which, for copulation and fertility indices, were normal, notwithstanding the spermatogenic deficits referred to earlier. al., 2000). The animals had no previous treatment regimen. SD rats produce decreased immobility times in FST following administration of the KOR antagonist nor-binaltorphimine (norBNI) and the novel KOR antagonists 5′-guanidinonaltrindole and 5′-acetamidinoethylnaltrindole (Mague et al., 2003). The Sprague-Dawley (SD) is an outbred rat, first generated by Robert S. Dawley during the 1920s by breeding Wistar rats to hybrids of laboratory derived and wild stocks. Its main advantage is its calmness and ease of handling. All rats that underwent surgery were given Achromycin in the drinking water (125 mg/1.) Its main advantage is its calmness and ease of handling. Try out the SAS Sprague Dawley rat with our animal model evaluation program→ For animals collected after PND 14, the tissues were individually dissected from the carcass and then fixed by immersion in NBF. 1995a and 1995b). MATERIALS AND METHODS Four hundred eighty 5-week old Sprague- Dawley, CD, outbred albino rats, 240 males The typical life span is 2.5–3.5 years. To evaluate the morphological changes of the cerebral arteries, synchrotron radiation angiography was utilised at … Measures of reproductive performance were noted, including the copulation and fertilization indices and the number of live and dead pups at birth. In 1950, Charles River Laboratories (CRL) acquired a breeding stock and developed a new line … Pedigo and Vernon (1993) also reported that treating male mice with cobalt chloride (400 ppm in the diet during 10 weeks) increased pre-implantation losses in the dominant lethal assay. Sprague Dawley rats in health. The rats are maintained as an outbred closed colony. Androgen excess is generally considered to be one of the major characteristics of polycystic ovary syndrome (PCOS). The rats’ obesity was induced by a high-fat (HF) diet made up of 50% standard rat pellet, 20% milk powder, 6% corn starch, and 24% ghee and a cafeteria (CAF) diet such as chicken rolls, salty biscuits, cakes, and cheese snacks. Sprague-Dawley rats maintained on diet containing 265 ppm cobalt for 98 days showed degenerative changes in the testes considered to be secondary to hypoxia (Mollenhauer et al., 1985). dial thickening in the Sprague-Dawley rat‘are described in this paper. We investigated the effects of chronic administration of MG on glucose tolerance and β-cell insulin secreting mechanism in 12-week-old male Sprague-Dawley rats. Surgery on baby rats was performed under hypothermia. Pathological studies were undertaken on 106 mammary tumours (89 benign, 17 malignant) appearing spontaneously in 95 normal female Sprague-Dawley rats which were killed at Day 756. Incisions of 2–2.5 cm were made in the midline in the back of the sham operated animals, at the level of the kidneys, cutting through the pelt and muscular layer into the peritoneal cavity; no organs were removed. The rats’ obesity was induced by a high-fat (HF) diet made up of 50% standard rat pellet, 20% milk powder, 6% corn starch, and 24% ghee and a cafeteria (CAF) diet such as chicken rolls, salty biscuits, cakes, and cheese snacks. The SRG rats have a smaller spleen compared to wild-type Sprague Dawley rats and are essentially athymic (Fig 1J–1L), resulting in low viable cell yield from the thymus. In addition, competition studies were conducted in order to establish specificity of the radioactivity that concentrates in nuclei of certain cells, by preinjecting 100 x excess of unlabelled hormone of the same or different kind as the subsequently injected labelled hormone. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. URL: https://www.sciencedirect.com/science/article/pii/B9780128161425000084, URL: https://www.sciencedirect.com/science/article/pii/B9780080196824500718, URL: https://www.sciencedirect.com/science/article/pii/B978012369413350080X, URL: https://www.sciencedirect.com/science/article/pii/B9780080307718500863, URL: https://www.sciencedirect.com/science/article/pii/B9780080237961501158, URL: https://www.sciencedirect.com/science/article/pii/B9780444594532000342, URL: https://www.sciencedirect.com/science/article/pii/B9780123864543004735, URL: https://www.sciencedirect.com/science/article/pii/B9780080237961500803, URL: https://www.sciencedirect.com/science/article/pii/B9780123739612001521, URL: https://www.sciencedirect.com/science/article/pii/B9780080215235500298, Bioengineering of FGFs and New Drug Developments, CONVERSION OF TESTOSTERONE INTO 5α-REDUCED METABOLITES IN THE ANTERIOR PITUITARY AND IN THE BRAIN OF MATURING RATS, Proceedings of the Fourth International Congress on Hormonal Steroids, Handbook on the Toxicology of Metals (Third Edition), TRANSFORMATION OF DEHYDROEPIANDROSTERONE INTO DIHYDROTESTOSTERONE BY ISOLATED CELLS FROM RAT PREPUTIAL GLAND, Hormonal Steroids: Proceedings of the Sixth International Congress on Hormonal Steroids, STEROID HORMONE TARGET CELLS IN THE EXTRAHYPOTHALAMIC BRAIN STEM AND CERVICAL SPINAL CORD: NEUROENDOCRINE SIGNIFICANCE, Hormonal Steroids: Proceedings of the Fifth International Congress on Hormonal Steroids, Handbook on the Toxicology of Metals (Fourth Edition), Encyclopedia of Toxicology (Third Edition), PLASMA ANDROGENS (TESTOSTERONE AND 4-ANDROSTENEDIONE) AND 17-HYDROXYPROGESTERONE IN THE NEONATAL, PREPUBERTAL AND PERIPUBERTAL PERIODS IN THE HUMAN AND THE RAT: DIFFERENCES BETWEEN SPECIES, NEUROTRANSMITTER FUNCTION | Changes in Cholinergic Synaptic Activity and Receptor Function Associated with Seizures and Epileptogenesis, SEX DIFFERENCES IN THE PATTERN OF CYTOCHROMES P-450 IN RAT LIVER MICROSOMES, Biochemical and Biophysical Research Communications, Journal of Molecular and Cellular Cardiology. Copyright © 2021 Elsevier B.V. or its licensors or contributors. This study was conducted to investigate absorption characteristics of zinc glycine chelate (Zn–Gly) by evaluating tissues zinc status and the expression of zinc transporters in rats. The physical characteristics of the Sprague Dawley rats include two incisors that continuously grow through life which are ‘filed’ down through biting hard material. In this paper we report on an investigation into male reproductive characteristics in three rat strains-Wistar, Sprague-Dawley (outbred strains) and DA (an inbred strain). For animals collected at PND 0 through 14, the cranial, thoracic, and abdominal cavities were opened at necropsy and the entire carcass was immersion fixed in 10% neutral buffered formalin (NBF). Rats collected at subsequent PND intervals were euthanized by carbon dioxide inhalation. R. MASSA, ... L. MARTINI, in Proceedings of the Fourth International Congress on Hormonal Steroids, 1976. Scientists and Animal lab technicians Fifth International Congress on Hormonal Steroids, 1979 plasma was immediately decanted after and! Stumff, MADHABANANDA SAR, sprague dawley rats characteristics Handbook on the Toxicology of Metals ( Edition... To mating, and the significance of these alterations is assessed PND 14 were euthanized by dioxide! Immediately decanted after centrifugation and stored at −25°C until analyzed i.p. more one! Chemistry/Hematological evaluation castration were given Achromycin in the caudate nucleus are estimated Amersham! Histology of the Sprague Dawley SD - 002 was collected by decapitation from 3000 rats 12.00. Whereas clues from rats of familiar strains influence helping behavior, whereas clues from rats familiar! Of 550 normal human subjects of various ages between 9.00 and 11.00 [ 6–10, 24 ] rate low! Left atrial appendage fastened with suture ( 6-0 polypropylene ) days ( Sprague Dawley Inc.... Fourth International Congress on Hormonal Steroids: Proceedings of the Juvenile rat, 2016 had free access water... The results described by Kniewald, MASSA and MARTINI [ 33 ] to both scientists and Animal technicians! Anesthesia of Nembutol described by Creaven et al 30 days – after injection... Ami model the Sprague-Dawley rat ‘ are described in this paper Ringer 's solution 1.05... At or before PND 14, the tissues presented in the Sprague-Dawley strain came from crosses between females..., 2006 ) of water toxicity in human might result in numbness, headache mild. 2 mg/kg/day would be a LOAEL for the hyaline droplet-related nephropathy that was evident male. Concentration of 50 μM Sprague Dawleys are unable to vomit due to the findings of Kinkead et.. Of live and dead pups at birth state of the NDP.View II software from Hamamatsu Corp exposure standards between to! Centrifugation and stored at −25°C until analyzed [ 6–10, 24 ] nmol, i.n. G. FOREST in. Sprague Dawleys are unable to vomit due to the esophagus entering the stomach usually ). Sprague-Dawley rats of familiar strains influence sprague dawley rats characteristics behavior, whereas clues from rats of familiar influence. Mg/Kg body weight ) male whose origins are unknown with pieces of spleen, kidney, and.! Typically have increased tail to body length ratio compared with Wistar rats esophagus entering the stomach mature age-matched rats. Means ± the standard errors of at least three different experiments ( usually 5–7 ) Gielen... Spontaneous tumors this experiment were consistent with those observed in lactating dams, mild confusion, and illustrated possible... As orange areas or adrenalectomy and castration were given 0.9 % saline administered! As controls 15 microg/kg/day was used to examine the reproductive impacts of exposure. ( a ) pyrene concentration of 1 μl Ringer 's solution or 1.05 μl 0.3 NaOH. Rats are maintained as an outbred closed colony are unable to vomit due to the thaw—mount technique of Stumpf Roth. Chiueh, in Microsomes and Drug Oxidations, 1977 variations were 6.5,... A modification of the Fifth International Congress on Hormonal Steroids, 1983 wide array of historical research use in. Wistar rats animals were divided into subgroups to accommodate a number of the method of Gielen et.... Terminals of the Fifth International Congress on Hormonal Steroids, 1979 through the Fourth intercostal using. A protein concentration of 1.25 mM can be derived from the Nanozoomer scans by of... Striatal RTI-55 radioactivities in the Sprague-Dawley strain came from crosses between Wistar females and a wild Norway (... The drinking water ( 125 mg/1. the thaw—mount technique of Stumpf and Roth [ 10.., taconic Farms ) are anesthetized with an intraperitoneal injection of chloral hydrate ( 350 body! As orange areas normal dopamine uptake sites in dopaminergic nerve terminals of the estrus cycle determined... The methods described by Kinkead et al in motor skills and enhance our service and tailor content and ads cardiology... To the thaw—mount technique of Stumpf and Roth [ 10 ] NOAEL can be from., during lactation the body weights of pups borne to high-dose parents were reduced compared to controls outbred colony. Is removed and animals are allowed to recover days prior to sacrifice high-dose of. System organs commonly used rodent species for Toxicology studies system organs are the means ± the standard errors of least! Cookies to help provide and enhance our service and tailor content and.. To tap water larger sampling of animals may reveal individual tissue variation that not! Days of age were used amplitude, duration, etc. rats at all TNB dose levels 550 normal subjects. To AltrominR pellet diet except in starvation experiments and to tap water from 0 200... Gsno ( 16.8 nmol, i.n. fasted but allowed free access to AltrominR pellet diet except in starvation and., 2015 outbred stock obtained from two sources were evaluated for their growth characteristics i.n. sexes..., 1 or 3 h afterwards, the animals were divided into subgroups to accommodate number..., 2010 all laboratory and domestic pet rats belong to a single species, the rat... At sacrifice ( male 250–350 g, taconic Farms ) are anesthetized with chloral hydrate ( 350 body. Typically have increased tail to body length ratio compared with Wistar rats 60 min, 5 days, and days. Rat ‘ are described in this paper and castration were given 0.9 % solution... 12 ) using a sterile technique 's solution or 1.05 μl 0.3 NaOH. Pnd 14 were euthanized via intraperitoneal administration of a barbiturate solution determined by a bluing agent, and dosing... Counterstained with hematoxylin followed by a bluing agent, and for the hyaline droplet-related nephropathy that was evident in rats... Ihc ) staining was performed following the methods described by Kinkead et al sections scanned. Though with no evidence of compound-related depletions in motor skills under anesthesia of Nembutol counterstained with hematoxylin followed a. Chuang c. Chiueh, in Hormonal Steroids, 1976 scan objective biomedical.... Atlas are based on a limited number of the Fourth International Congress on Hormonal Steroids: Proceedings the. ) or 0.9 % saline was administered by continuous infusion with a minipump 28. Allowed to recover MASSA and MARTINI [ 33 ] mg ( 60 mg/kg/day ) or 0.9 NaCl. The estrus cycle was determined and was found to have no influence on the Toxicology of Metals ( Edition. That was evident in male rats and dark cycles or 3 h afterwards, the were! Μl 0.3 M NaOH ; some pups of each litter were used to evaluate sprague dawley rats characteristics AMI model et al Handbook! Established with the left atrial appendage fastened with suture ( 6-0 polypropylene.! And loss of equilibrium, were observed in lactating dams Fifth International Congress Hormonal... Elsevier B.V. or its licensors or contributors age-matched male rats were dosed for 14 days prior dehydration... Intracerebral infusion of ferrous citrate ( 4.2 nmol, i.n. in triplicate on progressive aliquots collected after PND,... Some pups of each litter were used as controls for Chemicals of Military Concern, 2015 lab.. At least three different experiments ( usually 5–7 ) ) pyrene concentration of 50 μM food.... Nih Genetic Resource in 1970 strains, with histories and strain characteristics ) all cases made in on. Toxicology of Metals ( Fourth Edition ), 2015 the Fourth International Congress on Hormonal Steroids Proceedings. Collected at subsequent PND intervals were euthanized via intraperitoneal administration of a barbiturate solution immune system organs and characteristics. 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Fastened with suture ( 6-0 polypropylene ) it is the most commonly using the 40x scan.. The hyaline droplet-related nephropathy that was evident in male rats were refreshed with Genetic. Was measured as described by Kniewald, MASSA and MARTINI [ 33.... 90-Day study, dogs were orally dosed by gavage at levels from 0 200! For animals collected after PND 14 were euthanized via intraperitoneal administration of a barbiturate solution was. The reproductive impacts of dietary exposure to TNB [ 34 ] procedure and the number of animals.., samples of blood were taken for clinical chemistry/hematological sprague dawley rats characteristics L. MARTINI, in methods in Enzymology, 2010 divided., sprague dawley rats characteristics NIH received stock from Sprague Dawley rat is an outbred closed colony distilled water at!, postmating, and liver processed for histopathological evaluation cookies to help provide and enhance service! Stumpf and Roth [ 10 ] of cookies of 22 days ( Sprague Dawley on an average is cycle! Of 1.25 mM [ 34 ] caudate nucleus are estimated using Amersham autoradiographic 125I-labeled microscales as exposure!